ABSTRACT
In traditional Chinese medicine(TCM),abnormal and diseased conditions have been defined as Zheng Hou, a unique disease definition system in the context of holism. For over 3000 years the main clinical treatment method for TCM therapeutics has been so called Fang-ji, a TCM medicinal formula usually composed of several herbs and medical materials. The compositions of Fang-ji are based on the clinical practice under the guidelines of "bian-zheng-lun-zhi" and the principles of "Jun-chen-zuo-shi". Each Zheng is treated with a correspondingly-individualized Fang-ji.The modern approach to the study of Fang-ji pharmacology,however,has been focusing on the isolation and identification of individual active components for cellular and molecular targets. Although this approach has led to the development of many new monomers purified from Fang-ji as new drugs widely used in clinical practice such as the an-timalarial artemsinin,which has earned a Nobel Prize,the pharmacological bases of these purified effective monomers or active components have lost the TCM characteristics and are far different from the phar-macological theory and clinical applications of Fang-ji,in terms of the principles of"bian-zheng-lun-zhi"and "Jun-chen-zuo-shi". Here we introduce the emerging pharmacophenophenics as a systematical paradigm for the pharmacological study of Fang-ji.Pharmacophenomics studies the orchestrated multi-target pharmacology of combination therapy.With well-defined molecular mechanisms of Zheng Hou at the level of multi-omics and a suite of new phenomics technologies and platforms, the pharmacophe-nomics may be used to characterize the drug-response phenome of Fang-ji and to identify the corre-sponding multiple therapeutic targets according to the TCM theory of Jun-chen-zuo-shi.Pharmacophe-nomic study of Fang-ji will also lay a theoretical foundation for the new science of precision medicine.
ABSTRACT
<p><b>OBJECTIVE</b>To establish the migraine rheumatism stasis syndrome animal model.</p><p><b>METHOD</b>The rat migraine rheumatism stasis syndrome animal model was established through rheumatism stimulation with manual climate box, 5-HT reduction caused by reserpine and local cerebral vasospasm. General vital signs (activity, weight, eye gum, hair, feeding, excrement), head scratch frequency and image collection were observed to analyze the changes in biological signs of stasis syndrome (tongue image RGB), thrombin and serotonin of model rats.</p><p><b>RESULT</b>The reserpine group and the reserpine plus rheumatism model group showed significant reduction in blood coagulation time, pain threshold and 5-HT content in blood and brain (P < 0.01); the reserpine plus rheumatism model group showed an increase in eye gum and decreases in activity, feeding, with thin sloppy stool. According to the tough RGB values, the control group showed light red toughs, the reserpine group showed dark purple toughs, the reserpine plus rheumatism model group showed gray toughs, with notable differences in tough RGB values in all three group.</p><p><b>CONCLUSION</b>The rheumatism stimulation with manual climate box, 5-HT reduction caused by reserpine and local cerebral vasospasm can be used to induce the migraine rheumatism stasis syndrome animal model, but its modeling assessment method and process shall be further improved.</p>
Subject(s)
Animals , Female , Humans , Male , Rats , Blood Circulation , Diagnosis, Differential , Disease Models, Animal , Medicine, Chinese Traditional , Migraine Disorders , Diagnosis , Rats, Sprague-Dawley , Rheumatic Diseases , DiagnosisABSTRACT
Aerospace medicine has paid more and more attention to abnormal changes of physiological functions induced by weightlessness and studies on their prevention during space flight. In this paper, the effect of space weightlessness on cognitive functions was introduced. We tried to analyze the correlation between the cognitive function changes and relevant Chinese medical syndromes, thus providing a potential available way to prevent and treat weightlessness induced cognitive deficit during space flight.
Subject(s)
Humans , Aerospace Medicine , Cognition , Medicine, Chinese Traditional , WeightlessnessABSTRACT
<p><b>OBJECTIVE</b>To explore the distributional characteristics of acylation stimulating protein (ASP) gene polymorphism and the association with serum lipid level of Chinese Han and Uighur residents in Xinjiang.</p><p><b>METHOD</b>Genotypes of the ASP gene 301T > C polymorphism was detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) in 527 Uighur and 407 Han residents.</p><p><b>RESULTS</b>The frequencies of TT, TC and CC genotype of ASP gene 301T > C were 74.9%, 21.3% and 3.6% in Han group and 47.6, 40.2% and 12.1% in Uighur group (P < 0.05). Serum triglyceride level was significantly higher in C allele carrier (TC + CC genotype) than in TT genotype carrier of both Han and Uighur individuals. After adjusting for age, gender, smoking, drinking, BMI and serum total cholesterol, logistic regression analyses revealed that individuals carrying C allele (TC + CC genotype) faced an increased risk of increased serum triglyceride level than individuals carrying TT genotype in both Han and Uighur individuals (OR = 3.31, 95%CI: 1.31 - 8.36 in Uighur group; OR = 3.98, 95%CI: 1.81 - 8.74 in Han group).</p><p><b>CONCLUSION</b>There is a significant difference on mutational frequencies of the ASP gene 301T > C polymorphism between Uighur and Han residents in Xinjiang and C allele carriers face an increased risk for hypertriglyceridemia in both Uighur and Han residents in Xinjiang.</p>
Subject(s)
Female , Humans , Male , Middle Aged , Alleles , Complement C3 , Ethnicity , Genetics , Gene Frequency , Genotype , Intercellular Signaling Peptides and Proteins , Genetics , Polymorphism, Single Nucleotide , Triglycerides , BloodABSTRACT
<p><b>OBJECTIVE</b>To investigate the electrophysiological effect of Xin' an granule (XAG) on ventricular muscle cell in ischemic rabbits.</p><p><b>METHODS</b>A total of 48 rabbits were divided into the normal group and the ischemic group, and then subdivided into three groups, the control group, the high and low-dose XAG groups, 8 in each group. Rabbits in the low-dose XAG group and the high-dose XAG group were gastrogavaged XAG at the daily dose of 0. 85 g/kg and 3.40 g/kg, while the others in the control group were given the equal dosage of normal saline. All the rabbits were treated three times per day for successive 10 days. The rabbit model of ischemia was established by intravenous injected with 2. 5 U/kg posterior pituitary injection. Five minutes later, the monophasic action potential (MAP) and electrocardiogram (ECG) of each rabbit in the different groups were recorded and compared.</p><p><b>RESULTS</b>(1) To normal rabbits, XAG could significantly shorten the action 50% and 90% potential duration (APD)50 and APD90 of ventricular muscle cell (P < 0.05 ), and high-dose of XAG could significantly increased the Vmax of MAP(P <0. 05). (2) While to ischemic rabbits, XAG could significantly prolong APD50 and APD90, and significantly increased the action potential amplitude (APA) and Vmax of MAP (P < 0. 05).</p><p><b>CONCLUSION</b>(1) XAG can significantly shorten APD50 and APD90 of ventricular muscle cell, and high-dose XAG significantly increase the Vmax of MAP of normal rabbits. (2) XAG can delay and alleviate the manifestation characteristics of action potential of ventricular muscle cell during ischemia.</p>